1E8T

Structure of the multifunctional paramyxovirus hemagglutinin-neuraminidase

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	100
Detector technology	IMAGE PLATE
Spacegroup name	P 21 21 21
Unit cell lengths	71.710, 77.910, 198.160
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	6.000 - 2.500
R-factor	0.222
Rwork	0.222
R-free	0.27600
Structure solution method	MIR
RMSD bond length	0.007
RMSD bond angle	1.380
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000
High resolution limit [Å]	2.500
Rmerge	0.031	0.073 *
Total number of observations	420703 *
Number of reflections	38168
Completeness [%]	97.0	96 *
Redundancy	11

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	4.6		pH 4.60

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	PEG3350
2	1	reservoir	ammonium sulfate	0.2 (M)
3	1	reservoir	citrate	0.1 (M)