1E8O
Core of the Alu domain of the mammalian SRP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-2 |
| Synchrotron site | ESRF |
| Beamline | ID14-2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-09-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 57.448, 186.621, 189.824 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 47.460 - 3.200 |
| R-factor | 0.245 |
| Rwork | 0.245 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1914 MODIFIED |
| RMSD bond length | 0.008 |
| RMSD bond angle | 22.800 * |
| Data reduction software | MOSFLM (V. 6.0) |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.350 |
| High resolution limit [Å] | 3.200 | 3.220 |
| Rmerge | 0.104 | |
| Number of reflections | 16328 | |
| <I/σ(I)> | 5.6 | 1.5 |
| Completeness [%] | 94.9 | 95.1 |
| Redundancy | 2.4 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 5 | 12 * | 50MM NAOAC, 10MM MGCL2, 140MM NACL, 390MM (NH4)2SO4, 21% PEG2000, pH 5.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | SA50 | 0.130 (mM) | |
| 10 | 1 | reservoir | sodium acetate | 50 (mM) | pH5.0 |
| 11 | 1 | reservoir | 10 (mM) | ||
| 12 | 1 | reservoir | 140 (mM) | ||
| 13 | 1 | reservoir | ammonium sulfate | 390 (mM) | |
| 14 | 1 | reservoir | PEG2000 | 21 (%) | |
| 2 | 1 | drop | SRP9/14 | 0.180 (mM) | |
| 3 | 1 | drop | HEPES | 2.5 (mM) | pH7.5 |
| 4 | 1 | drop | sodium acetate | 25 (mM) | pH5.0 |
| 5 | 1 | drop | 10 (mM) | ||
| 6 | 1 | drop | 75 (mM) | ||
| 7 | 1 | drop | ammonium sulfate | 210 (mM) | |
| 8 | 1 | drop | dithiothreitol | 5 (mM) | |
| 9 | 1 | drop | PEG2000 | 11 (%) |
