1E8I
HUMAN CD69 - TETRAGONAL FORM
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ESRF BEAMLINE ID14-3 |
Synchrotron site | ESRF |
Beamline | ID14-3 |
Temperature [K] | 100 |
Detector technology | CCD |
Detector | MARRESEARCH |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 69.629, 69.629, 118.588 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 25.000 - 1.950 |
R-factor | 0.248 |
Rwork | 0.248 |
R-free | 0.27000 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1b6e |
RMSD bond length | 0.007 |
RMSD bond angle | 22.400 * |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS (1.0) |
Refinement software | CNS (1.0) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.000 |
High resolution limit [Å] | 1.950 | 1.950 |
Rmerge | 0.061 * | 0.202 * |
Total number of observations | 98431 * | |
Number of reflections | 21836 | |
<I/σ(I)> | 27.5 | 5.5 |
Completeness [%] | 99.1 | 99.2 |
Redundancy | 5.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | Vapor diffusion * | 7.2 * | drop consists of equal amounts of protein and reservoir solutions * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 5 (mg/ml) | |
2 | 1 | drop | HEPES | 15 (mM) | |
3 | 1 | drop | 50 (mM) | ||
4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
5 | 1 | reservoir | zinc sulfate | 150 (mM) | or sodium sulfate |
6 | 1 | reservoir | PEG6000 | 15 (%) |