1E7P
QUINOL:FUMARATE REDUCTASE FROM WOLINELLA SUCCINOGENES
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 275 |
| Detector technology | CCD |
| Collection date | 2000-07-09 |
| Detector | MARRESEARCH |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 81.074, 290.240, 153.614 |
| Unit cell angles | 90.00, 95.73, 90.00 |
Refinement procedure
| Resolution | 30.000 - 3.100 |
| R-factor | 0.283 |
| Rwork | 0.283 |
| R-free | 0.29100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1QLA |
| RMSD bond length | 0.012 |
| RMSD bond angle | 22.500 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 3.290 |
| High resolution limit [Å] | 3.100 | 3.100 |
| Number of reflections | 102882 | |
| <I/σ(I)> | 11.9 | 2 |
| Completeness [%] | 80.8 | 61.7 |
| Redundancy | 2.7 | 1.97 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 6.4 * | used microseeding, Lancaster, C.R.D., (1999) Nature, 402, 377. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | 1 (mM) | ||
| 10 | 1 | drop | HEPES | 10 (mM) | |
| 11 | 1 | drop | citrate | 10 (mM) | pH6.4 |
| 12 | 1 | drop | protein | 9.5 (mg/ml) | |
| 13 | 1 | reservoir | 150 (mM) | ||
| 14 | 1 | reservoir | PEG3350 | 10 (%) | |
| 15 | 1 | reservoir | citrate | 20 (mM) | pH5.6 |
| 2 | 1 | drop | dodecyl-beta-D-maltoside | 0.05 (%) | |
| 3 | 1 | drop | decylmaltoside | 0.20 (%) | |
| 4 | 1 | drop | benzamidine | 2.4 (%) | |
| 5 | 1 | drop | PEG3350 | 5 (%) | |
| 6 | 1 | drop | 75 (mM) | ||
| 7 | 1 | drop | DME | 5 (%) | |
| 8 | 1 | drop | DMN | 1 (mM) | |
| 9 | 1 | drop | malonate | 1 (mM) |
