1E6M
TWO-COMPONENT SIGNAL TRANSDUCTION SYSTEM D57A MUTANT OF CHEY
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 287 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 45.800, 47.700, 54.100 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.700 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.26000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.575 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | |
| High resolution limit [Å] | 1.700 | 2.000 * |
| Rmerge | 0.051 | |
| Number of reflections | 8189 | |
| Completeness [%] | 90.8 | 84.2 * |
| Redundancy | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 4 * | pH 7.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.8 (mg/ml) | |
| 2 | 1 | drop | PEG8000 | 12.5 (%) | |
| 3 | 1 | drop | cacodylate | 50 (mM) | |
| 4 | 1 | reservoir | PEG8000 | 25 (%) | |
| 5 | 1 | reservoir | cacodylate | 100 (mM) |
