1E6J
Crystal structure of HIV-1 capsid protein (p24) in complex with Fab13B5
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-3 |
| Synchrotron site | ESRF |
| Beamline | ID14-3 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1998-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 193.080, 45.600, 132.470 |
| Unit cell angles | 90.00, 132.41, 90.00 |
Refinement procedure
| Resolution | 15.000 - 3.000 |
| R-factor | 0.21 |
| Rwork | 0.210 |
| R-free | 0.28400 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | FAB13B5 1afv 1AM3 |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.201 |
| Data reduction software | MOSFLM |
| Data scaling software | CCP4 |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.8) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.700 | |
| High resolution limit [Å] | 3.000 | 3.000 |
| Rmerge | 0.094 * | 0.276 * |
| Number of reflections | 14742 | |
| Completeness [%] | 84.7 | 33.1 |
| Redundancy | 2.4 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | unknown * | 7.5 | 277 | PROTEIN AT 7MG/ML IN 7% PEG8000, 0.1M TRIS-HCL PH=7.5, 5MM DTT, 0.5MM K2PTCL4 AT 4C USING THE HANGING DROP SYSTEM, pH 7.50 |
