1E65
Azurin from Pseudomonas aeruginosa, apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 288 |
| Detector technology | DIFFRACTOMETER |
| Collection date | 1991-01-10 |
| Detector | ENRAF-NONIUS FAST |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 57.090, 81.100, 110.970 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 1.850 |
| R-factor | 0.193 |
| Rwork | 0.193 |
| Structure solution method | OTHER |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.940 |
| Data reduction software | MADNESS |
| Data scaling software | ABSCOR |
| Phasing software | X-PLOR (2.1) |
| Refinement software | X-PLOR (2.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.900 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.089 | |
| Total number of observations | 141499 * | |
| Number of reflections | 39493 | |
| Completeness [%] | 81.1 | 20.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 5.5 | PH 5.5 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | ammonium sulfate | 2.0 (M) | |
| 3 | 1 | drop | lithium nirate | 0.3 (M) | |
| 4 | 1 | reservoir | ammonium sulfate | 3.2 (M) | |
| 5 | 1 | reservoir | lithium nirate | 0.5 (M) | |
| 6 | 1 | reservoir | acetate | 0.1 (M) |
