1E63
Ferredoxin:NADP+ Reductase Mutant with LYS 75 Replaced by SER (K75S)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ENRAF NONIUS |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2000-01-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 65 |
| Unit cell lengths | 88.100, 88.100, 97.170 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 15.000 - 2.300 |
| R-factor | 0.19 |
| Rwork | 0.190 |
| R-free | 0.24000 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1que |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.854 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | X-PLOR (3.843) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 35.500 | 2.430 |
| High resolution limit [Å] | 2.300 | 2.310 |
| Rmerge | 0.103 | 0.312 |
| Number of reflections | 18750 | |
| <I/σ(I)> | 6.7 | 2.4 |
| Completeness [%] | 99.4 | 95.8 |
| Redundancy | 7.9 | 6.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | 20 * | pH 5.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 0.75 (mM) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | beta-octylglucoside | 5 (%(w/v)) | |
| 4 | 1 | reservoir | PEG6000 | 18-20 (%(w/v)) | |
| 5 | 1 | reservoir | ammonium sulfate | 20 (mM) | |
| 6 | 1 | reservoir | MES- NaOH | 0.1 (M) |
