1E5W
Structure of isolated FERM domain and first long helix of moesin
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-15 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.87, 1.3 |
| Spacegroup name | P 42 21 2 |
| Unit cell lengths | 94.360, 94.360, 156.050 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 37.120 - 2.700 |
| R-factor | 0.254 |
| Rwork | 0.254 |
| R-free | 0.29700 |
| Structure solution method | MAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 22.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | SOLVE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.000 | 2.850 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rmerge | 0.046 | 0.246 |
| Total number of observations | 110406 * | |
| Number of reflections | 19994 | 2805 * |
| <I/σ(I)> | 4.6 | 3 |
| Completeness [%] | 99.7 | 99.9 |
| Redundancy | 5.5 | 5.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 | 16 * | 25% ETHYLENE GLYCOL, 100MM HEPES PH7.5, 10-15 MG/ML PROTEIN, pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10-15 (mg/ml) | |
| 2 | 1 | reservoir | HEPES | 100 (mM) | |
| 3 | 1 | reservoir | ethylene glycol | 25 (%) |
