1E5W

Structure of isolated FERM domain and first long helix of moesin

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-10-15
Detector	MARRESEARCH
Wavelength(s)	0.87, 1.3
Spacegroup name	P 42 21 2
Unit cell lengths	94.360, 94.360, 156.050
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.120 - 2.700
R-factor	0.254
Rwork	0.254
R-free	0.29700
Structure solution method	MAD
RMSD bond length	0.007
RMSD bond angle	22.200 *
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	SOLVE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.000	2.850
High resolution limit [Å]	2.700	2.700
Rmerge	0.046	0.246
Total number of observations	110406 *
Number of reflections	19994	2805 *
<I/σ(I)>	4.6	3
Completeness [%]	99.7	99.9
Redundancy	5.5	5.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.5	16 *	25% ETHYLENE GLYCOL, 100MM HEPES PH7.5, 10-15 MG/ML PROTEIN, pH 7.50

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10-15 (mg/ml)
2	1	reservoir	HEPES	100 (mM)
3	1	reservoir	ethylene glycol	25 (%)