1E5S

Proline 3-hydroxylase (type II) - Iron form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX7.2
Synchrotron site	SRS
Beamline	PX7.2
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-11-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	72.780, 72.780, 224.900
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	23.820 - 2.400
R-factor	0.198 *
Rwork	0.216
R-free	0.22600 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	PROLINE HYDROXYLASE APO FORM
RMSD bond length	0.047 *
RMSD bond angle	3.000 *
Data reduction software	DENZO
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	24.000 *	2.360
High resolution limit [Å]	2.400 *	2.400 *
Rmerge	0.101 *	0.400 *
Total number of observations	375824 *
Number of reflections	26230 *
<I/σ(I)>	3	1.1
Completeness [%]	93.8 *	98.4
Redundancy	2.9	3.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	21 *	2M AMMONIUM SULFATE, 50MM MES BUFFER, PH6, pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	MES	50 (mM)	pH6.0
3	1	reservoir	HEPES	50 (mM)	pH7.5
4	1	reservoir	PEG400	2 (%)
5	1	reservoir	ammonium sulfate	2.0 (M)