1E5R

Proline 3-hydroxylase (type II) -apo form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ESRF BEAMLINE BM14
Synchrotron site	ESRF
Beamline	BM14
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-02-15
Detector	MARRESEARCH
Spacegroup name	P 31 2 1
Unit cell lengths	72.540, 72.540, 223.920
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	28.530 - 2.300
R-factor	0.202 *
Rwork	0.225
R-free	0.25300 *
Structure solution method	MIR
RMSD bond length	0.044 *
RMSD bond angle	2.700 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHARP
Refinement software	CNS (1.0)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.370
High resolution limit [Å]	2.300 *	2.300 *
Rmerge	0.052	0.275
Total number of observations	357708 *
Number of reflections	31128 *
<I/σ(I)>	12.6
Completeness [%]	98.7	95.4
Redundancy	3.1	2.94

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	21 *	2M AMMONIUM SULFATE, 50MM MES BUFFER, PH6, pH 6.00

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15 (mg/ml)
2	1	drop	MES	50 (mM)	pH6.0
3	1	reservoir	HEPES	50 (mM)	pH7.5
4	1	reservoir	PEG400	2 (%)
5	1	reservoir	ammonium sulfate	2.0 (M)