1E5R
Proline 3-hydroxylase (type II) -apo form
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 72.540, 72.540, 223.920 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 28.530 - 2.300 |
| R-factor | 0.202 * |
| Rwork | 0.225 |
| R-free | 0.25300 * |
| Structure solution method | MIR |
| RMSD bond length | 0.044 * |
| RMSD bond angle | 2.700 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | SHARP |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 * | 2.370 |
| High resolution limit [Å] | 2.300 * | 2.300 * |
| Rmerge | 0.052 | 0.275 |
| Total number of observations | 357708 * | |
| Number of reflections | 31128 * | |
| <I/σ(I)> | 12.6 | |
| Completeness [%] | 98.7 | 95.4 |
| Redundancy | 3.1 | 2.94 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 21 * | 2M AMMONIUM SULFATE, 50MM MES BUFFER, PH6, pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 15 (mg/ml) | |
| 2 | 1 | drop | MES | 50 (mM) | pH6.0 |
| 3 | 1 | reservoir | HEPES | 50 (mM) | pH7.5 |
| 4 | 1 | reservoir | PEG400 | 2 (%) | |
| 5 | 1 | reservoir | ammonium sulfate | 2.0 (M) |
