1E5M
Beta ketoacyl acyl carrier protein synthase II (KASII) from Synechocystis sp.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 100.800, 100.800, 74.700 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 20.000 - 1.540 |
| R-factor | 0.175 |
| Rwork | 0.175 |
| R-free | 0.19900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1kas |
| RMSD bond length | 0.004 |
| RMSD bond angle | 22.900 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.5) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.058 * | 0.187 * |
| Number of reflections | 63411 | |
| <I/σ(I)> | 17.9 | 2.3 |
| Completeness [%] | 97.5 | 90.9 |
| Redundancy | 5.3 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | pH 5.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 14 (mg/ml) | |
| 2 | 1 | drop | imidazole | 75 (mM) | |
| 3 | 1 | drop | Tris | 25 (mM) | |
| 4 | 1 | drop | 300 (mM) | ||
| 5 | 1 | reservoir | PEG4000 | 22 (%(w/v)) | |
| 6 | 1 | reservoir | tri-sodium citrate | 0.085 (M) | |
| 7 | 1 | reservoir | ammonium acetate | 0.170 (M) | |
| 8 | 1 | reservoir | glycerol | 15 (%(v/v)) | |
| 9 | 1 | reservoir | 1,2,3 heptanetriol | 15 (%(w/v)) |
