1E5L
Apo saccharopine reductase from Magnaporthe grisea
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I711 |
| Synchrotron site | MAX II |
| Beamline | I711 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-03-15 |
| Detector | MARRESEARCH |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 89.330, 119.000, 195.940 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.910 - 2.400 |
| R-factor | 0.222 |
| Rwork | 0.222 |
| R-free | 0.25900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ff9 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 23.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | EPMR |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 2.440 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.090 | 0.335 |
| Total number of observations | 386685 * | |
| Number of reflections | 40017 | |
| <I/σ(I)> | 24 | 3.3 |
| Completeness [%] | 97.1 | 82.2 |
| Redundancy | 9.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 4 | 4 * | 8 MG/ML PROTEIN, 11-14 % (W/W) PEG6000, 0.1 M CITRIC ACID PH 4.0, 0.1 % BETA-OCTYLGLUCOSIDE, 1 MM DTT |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | beta-octylglucoside | 0.5 (%) | |
| 3 | 1 | reservoir | PEG6000 | 11-14 (%(w/w)) | |
| 4 | 1 | reservoir | citric acid | 0.1 (M) | |
| 5 | 1 | reservoir | dithiothreitol | 1 (mM) |
