1E5E
METHIONINE GAMMA-LYASE (MGL) FROM TRICHOMONAS VAGINALIS IN COMPLEX WITH PROPARGYLGLYCINE
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-06-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 31 1 2 |
| Unit cell lengths | 88.258, 88.258, 217.849 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 25.000 - 2.180 |
| Rwork | 0.140 |
| R-free | 0.19300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1cl1 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 0.032 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 28.900 | 2.260 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 48724 | |
| <I/σ(I)> | 23.5 | 6.05 |
| Completeness [%] | 97.6 | 77.4 |
| Redundancy | 3.6 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | 5.6 | 3.2M AMMONIUM SULPHATE, 0.2M LISO4, 0.1M CITRATE PH5.6, pH 5.60 |
