1E4J

Crystal structure of the soluble human Fc-gamma Receptor III

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RU200
Temperature [K]	291
Detector technology	IMAGE PLATE
Collection date	1999-09-15
Detector	MARRESEARCH
Spacegroup name	P 2 21 21
Unit cell lengths	36.700, 60.290, 85.580
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 2.500
R-factor	0.1951
Rwork	0.195
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2fcb
RMSD bond length	0.012
RMSD bond angle	1.640
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	AMoRE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.500
High resolution limit [Å]	2.500	2.300
Rmerge	0.114	0.500 *
Number of reflections	6725
Completeness [%]	94.7	72.3 *
Redundancy	3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	7.1 *	18 *	0.1M MES/TRIS PH 7.8, 22.0% PEG8000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	Mops	2 (mM)
3	1	drop		150 (mM)
4	1	drop	sodium azide	0.02 (%)
5	1	reservoir	MES-Tris	100 (mM)
6	1	reservoir	PEG8000	6 (%)
7	1	reservoir	K-Na tartrate	200 (mM)
8	1	reservoir	sodium azide	0.02 (%)