1E4G
FtsA (ATP-bound form) from Thermotoga maritima
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Collection date | 2000-02-15 |
| Wavelength(s) | 0.9393, 0.9787, 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 50.450, 73.740, 57.040 |
| Unit cell angles | 90.00, 113.46, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.600 |
| R-factor | 0.22 |
| Rwork | 0.220 |
| R-free | 0.28200 |
| Structure solution method | MAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.376 |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.740 |
| High resolution limit [Å] | 2.600 | 2.600 |
| Rmerge | 0.066 | 0.235 |
| Number of reflections | 11678 | |
| <I/σ(I)> | 18.1 | 5.9 |
| Completeness [%] | 97.9 | 98.1 |
| Redundancy | 6.2 | 5.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.5 | 18 * | 200 MM AMMONIUM ACETATE, 100 MM NA ACETATE PH 5.5, 15% PEG |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | drop | ADP | 1 (mM) | |
| 3 | 1 | drop | 2 (mM) | ||
| 4 | 1 | reservoir | ammonium acetate | 200 (mM) | |
| 5 | 1 | reservoir | sodium acetate | 100 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 15 (%) |
