1E4F
FtsA (apo form) from Thermotoga maritima
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE ID14-4 |
| Synchrotron site | ESRF |
| Beamline | ID14-4 |
| Temperature [K] | 100 |
| Collection date | 2000-02-15 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.620, 53.520, 78.310 |
| Unit cell angles | 90.00, 93.44, 90.00 |
Refinement procedure
| Resolution | 30.000 - 1.900 |
| R-factor | 0.221 |
| Rwork | 0.221 |
| R-free | 0.24900 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 1.197 |
| Phasing software | CNS (1.0) |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 6.000 | 2.000 |
| High resolution limit [Å] | 1.900 | 1.900 |
| Rmerge | 0.053 * | 0.164 |
| Number of reflections | 30124 | |
| <I/σ(I)> | 5.3 | 5.3 |
| Completeness [%] | 94.8 | 76.8 |
| Redundancy | 2.5 | 2.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, sitting drop * | 5.6 | 18 * | 300 MM KCL, 50 MM MES, 5.6, 5% PEG6000, 5-15% PEG400, pH 5.60 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 13 (mg/ml) | |
| 2 | 1 | reservoir | 300 (mM) | ||
| 3 | 1 | reservoir | PEG6000 | 5 (%) | |
| 4 | 1 | reservoir | MES | 50 (mM) |
