1E4A
L-Fuculose 1-Phosphate Aldolase from Escherichia coli Mutant Del(27)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU RUH2R |
Temperature [K] | 293 |
Detector technology | AREA DETECTOR |
Collection date | 1998-01-15 |
Detector | MULTIWIRE SIEMENS X-100 |
Spacegroup name | P 4 21 2 |
Unit cell lengths | 93.250, 93.250, 43.400 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 10.000 - 2.150 |
R-factor | 0.156 * |
Rwork | 0.156 |
R-free | 0.22300 |
Structure solution method | FOURIER SYNTHESIS |
RMSD bond length | 0.010 |
RMSD bond angle | 0.034 |
Data reduction software | XDS |
Refinement software | REFMAC |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 10.000 | 2.220 |
High resolution limit [Å] | 2.150 | 2.150 |
Rmerge | 0.068 * | 0.190 * |
Number of reflections | 9818 | 795 * |
<I/σ(I)> | 9.7 | 4 |
Completeness [%] | 92.0 | 82 |
Redundancy | 4.9 | 2.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.6 * | 293 * | Dreyer, M.K., (1996) Acta Crystallog. sect., D52, 1082. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 10 (mg/ml) | |
2 | 1 | drop | Tris-HCl | 20 (mM) | |
3 | 1 | drop | 0.5 (mM) | ||
4 | 1 | drop | beta-mercaptoethanol | 10 (mM) | |
5 | 1 | reservoir | ammonium sulfate | 1.62 (M) | |
6 | 1 | reservoir | potassium phosphate | 20 (mM) | |
7 | 1 | reservoir | 4 (mM) | ||
8 | 1 | reservoir | beta-mercaptoethanol | 4 (mM) |