1E2Z
Q158L mutant of cytochrome f from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-BM-C |
| Synchrotron site | APS |
| Beamline | 14-BM-C |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-15 |
| Detector | ADSC CCD |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 73.950, 94.660, 122.640 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 44.160 - 2.500 |
| R-factor | 0.218 |
| Rwork | 0.218 |
| R-free | 0.27900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1e2v |
| RMSD bond length | 0.007 |
| RMSD bond angle | 25.300 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 47.140 | 2.590 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.078 | 0.201 |
| Total number of observations | 162713 * | |
| Number of reflections | 29632 | |
| <I/σ(I)> | 22.7 | 4.8 |
| Completeness [%] | 97.1 | 73.1 |
| Redundancy | 5.5 | 2.34 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | THE PROTEIN WAS BUFFERED IN 10 MM NA2HPO4/NAH2PO4, 1 MM DTT, PH 7.5 AND THE RESERVOIR CONTAINED 100 MM MES, PH 6.7, 50 MM AMMONIUM FLUORIDE 5% GLYCEROL AND 19-21% PEG 3350. |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 10 (mM) | ||
| 3 | 1 | drop | dithiothreitol | ||
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | ammonium fluoride | 50 (mM) | |
| 6 | 1 | reservoir | glycerol | 5 (%) | |
| 7 | 1 | reservoir | PEG3350 | 19-21 (%) |
