1E2V
N153Q mutant of cytochrome f from Chlamydomonas reinhardtii
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 14-ID-B |
| Synchrotron site | APS |
| Beamline | 14-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-04-15 |
| Detector | ADSC QUANTUM 4 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 76.120, 94.220, 121.080 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 42.330 - 1.850 |
| R-factor | 0.185 |
| Rwork | 0.185 |
| R-free | 0.21900 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ewh |
| RMSD bond length | 0.005 |
| RMSD bond angle | 25.600 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | AMoRE |
| Refinement software | CNS (1.0) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.850 | 1.920 |
| High resolution limit [Å] | 1.850 | 1.850 |
| Rmerge | 0.048 | 0.305 |
| Total number of observations | 329989 * | |
| Number of reflections | 73484 | |
| <I/σ(I)> | 26.1 | 3.73 |
| Completeness [%] | 97.9 | 90.2 |
| Redundancy | 4.5 | 3.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.5 * | 12 * | temperature was changed to 20 degress after 4 days * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 80 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | reservoir | MES | 100 (mM) | |
| 5 | 1 | reservoir | calcium acetate | 25 (mM) | |
| 6 | 1 | reservoir | dithiothreitol | 1 (mM) | |
| 7 | 1 | reservoir | PEG8000 | 17-19 (%) |
