1E29

PSII associated cytochrome C549 from Synechocystis sp.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE BW7B
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	BW7B
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-10-15
Detector	MARRESEARCH
Spacegroup name	C 2 2 21
Unit cell lengths	34.220, 86.860, 97.660
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	97.700 - 1.210
R-factor	0.1486
R-free	0.21240
Structure solution method	DIRECT METHODS
RMSD bond length	0.013
RMSD bond angle	0.034
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SHELXD
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	97.700	1.230
High resolution limit [Å]	1.210	1.210
Rmerge	0.040	0.338
Number of reflections	42933
<I/σ(I)>	31.1	2.8
Completeness [%]	95.8	85.9
Redundancy	4.9	3.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5		SITTING DROP, VAPOUR DIFFUSION, 16% PEG 8000, O.2 M CALCIUM ACETATE, 0.1 M TRIS/HCL, PH = 8.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	PEG8000	16 (%)
2	1	drop	calcium acetate	0.2 (M)
3	1	drop	Tris-HCl	0.1 (M)
4	1	drop	protein	30 (mg/ml)