1E0D
UDP-N-Acetylmuramoyl-L-Alanine:D-Glutamate Ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE D2AM |
| Synchrotron site | ESRF |
| Beamline | D2AM |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1997-10-23 |
| Detector | MARRESEARCH |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 69.160, 69.160, 196.710 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.400 |
| R-factor | 0.213 |
| Rwork | 0.213 |
| R-free | 0.26200 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2 DOMAINS FROM 1UAG |
| RMSD bond length | 0.016 |
| RMSD bond angle | 24.300 * |
| Data reduction software | XDS |
| Phasing software | CNS (0.4) |
| Refinement software | CNS (0.4) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.088 * | 0.212 * |
| Number of reflections | 19294 | |
| <I/σ(I)> | 13 | 3.7 |
| Completeness [%] | 98.9 * | 99.9 |
| Redundancy | 7.3 | 6.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.5 * | 15 * | drop solution was mixed with an equal volume of reservoir solution * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 9.8 (mg/ml) | |
| 2 | 1 | drop | 1 (mM) | ||
| 3 | 1 | drop | dithiothreitol | 1 (mM) | |
| 4 | 1 | drop | HEPES | 20 (mM) | |
| 5 | 1 | reservoir | ammonium sulfate | 1.5-1.7 (M) | |
| 6 | 1 | reservoir | MES | 100 (mM) | |
| 7 | 1 | reservoir | MPD | 5 (%(v/v)) |
