1E00

Porcine Odorant Binding Protein Complexed with 2,6-dimethyl-7-octen-2-ol

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.076, 88.287, 93.588
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.830
Rwork	0.217
R-free	0.25800
Structure solution method	OTHER
RMSD bond length	0.015
RMSD bond angle	1.420 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.860
High resolution limit [Å]	1.830	1.830
Rmerge	0.092 *	0.162 *
Number of reflections	30278
<I/σ(I)>	11.5	3.85
Completeness [%]	96.1	96.9
Redundancy	2.9 *	2.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	20 *	2M AMMONIUM SULFATE, AND 5% ISOPROPANOL, pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	iso-propanol	5 (%(v/v))