1DZX
L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant R212A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | SIEMENS XP-18H |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 1995-04-15 |
| Detector | SIEMENS X-1000 CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 93.700, 93.700, 42.600 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.180 |
| Rwork | 0.146 |
| R-free | 0.20000 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.032 |
| Data reduction software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 2.240 |
| High resolution limit [Å] | 2.180 | 2.180 |
| Number of reflections | 9269 | |
| Completeness [%] | 89.0 | 65 |
| Redundancy | 3.9 | 1.9 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | CRYSTALS GROWN FROM AMMONIUM SULFATE AT PH 8.0,VAPOUR DIFFUSION, HANGING DROP,CONDITIONS CLOSE TO THE ONES REPORTED FOR THE WILD-TYPE, SEE PDB ID 1FUA FOR FURTHER DETAILS |
