1DZW

L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant F131A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	AREA DETECTOR
Collection date	1996-12-14
Detector	MULTIWIRE SIEMENS
Spacegroup name	P 4 21 2
Unit cell lengths	93.900, 93.900, 43.300
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.170
Rwork	0.152
R-free	0.21800
Structure solution method	OTHER
RMSD bond length	0.010
RMSD bond angle	0.032
Data reduction software	XDS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.240
High resolution limit [Å]	2.170	2.170
Number of reflections	10014
<I/σ(I)>	10.1	5.4
Completeness [%]	95.0	87
Redundancy	3.2	1.8

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		CRYSTALS GROWN FROM AMMONIUM SULFATE AT PH 8.0,VAPOUR DIFFUSION, HANGING DROP, CONDITIONS CLOSE TO THE ONES REPORTED FOR THE WILD-TYPE, SEE PDB ID 1FUA FOR FURTHER DETAILS