1DZV
L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant Y113F/Y209F
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RUH2R |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 1995-11-15 |
| Detector | SIEMENS X-1000 CCD |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 93.700, 93.700, 42.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 1.860 |
| Rwork | 0.168 |
| R-free | 0.21600 |
| Structure solution method | OTHER |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.027 |
| Data reduction software | XDS |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 10.000 | 1.920 |
| High resolution limit [Å] | 1.860 | 1.860 |
| Number of reflections | 15024 | |
| <I/σ(I)> | 8.5 | 4.4 |
| Completeness [%] | 92.0 | 69 |
| Redundancy | 4.9 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | CRYSTALS GROWN FROM AMMONIUM SULFATE AT PH 8.0, VAPOUR DIFFUSION, HANGING DROP, CONDITIONS CLOSE TO THE ONES REPORTED FOR THE WILD-TYPE, SEE PDB ID 1FUA FOR FURTHER DETAILS |
