1DZU

L-Fuculose-1-Phosphate Aldolase from Escherichia coli Mutant T26A

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH2R
Temperature [K]	293
Detector technology	CCD
Collection date	1996-02-15
Detector	SIEMENS X-1000 CCD
Spacegroup name	P 4 21 2
Unit cell lengths	93.800, 93.800, 43.000
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	10.000 - 2.090
Rwork	0.150
R-free	0.20700
Structure solution method	OTHER
RMSD bond length	0.009
RMSD bond angle	0.031
Data reduction software	XDS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	10.000	2.160
High resolution limit [Å]	2.090	2.090
Rmerge	0.066 *	0.150 *
Number of reflections	10121	822 *
<I/σ(I)>	8.6	4.8
Completeness [%]	87.0	78
Redundancy	3.1	2.3

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8		CRYSTALS GROWN FROM AMMONIUM SULFATE AT PH 8.0,VAPOUR DIFFUSION, HANGING DROP, CONDITIONS CLOSE TO THE ONES REPORTED FOR THE WILD-TYPE, SEE PDB ID 1FUA FOR FURTHER DETAILS