1DZQ

LECTIN UEA-II COMPLEXED WITH GALACTOSE

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X11
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X11
Temperature [K]	287
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 32 2 1
Unit cell lengths	104.870, 104.870, 175.300
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	16.000 - 2.850
R-factor	0.186
Rwork	0.186
R-free	0.20900
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qot
RMSD bond length	0.007
RMSD bond angle	27.143 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	16.000	3.000
High resolution limit [Å]	2.850	2.850
Rmerge	0.193 *	0.686 *
Total number of observations	169762 *
Number of reflections	26439	2606 *
<I/σ(I)>	10.97	3.51
Completeness [%]	99.8	100
Redundancy	6.42	6.35

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5		Dao-Thi M.H., (1998) Acta Crystallog. sect., D54, 844. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	2.0-5.0 (mg/ml)
2	1	reservoir	cacodylate	100 (mM)	or MES
3	1	reservoir	PEG6000	8-16 (%(w/v))