1DZJ

Porcine Odorant Binding Protein Complexed with 2-amino-4-butyl-5-propylselenazole

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LURE BEAMLINE DW32
Synchrotron site	LURE
Beamline	DW32
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1997-12-01
Detector	MARRESEARCH
Spacegroup name	P 21 21 21
Unit cell lengths	42.362, 88.739, 93.197
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.000
R-factor	0.229
Rwork	0.229
R-free	0.24300
Structure solution method	OTHER
RMSD bond length	0.020 *
RMSD bond angle	26.300 *
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	CNS (0.5)
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.900
High resolution limit [Å]	1.900	1.860
Rmerge	0.065 *	0.250 *
Number of reflections	25700
<I/σ(I)>	9	1.92
Completeness [%]	96.1	97.2
Redundancy	3	2.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	7.8	20 *	PROTEIN (0.47MM) IS INCUBATED WITH 10MM OF SELENAZOLE, THE COMPLEX WAS CRYSTALLISED FROM 2M AMMONIUM SULFATE, AND 5% ISOPROPANOL IN HANGING DROPS, AT 20 DEGREES., pH 7.80

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	reservoir	ammonium sulfate	2 (M)
3	1	reservoir	iso-propanol	5 (%(v/v))