1DZF

RPB5 from S.cerevisiae

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SRS BEAMLINE PX9.6
Synchrotron site	SRS
Beamline	PX9.6
Temperature [K]	100
Detector technology	CCD
Collection date	1999-05-15
Detector	ADSC QUANTUM 4
Spacegroup name	C 2 2 21
Unit cell lengths	45.050, 82.220, 134.810
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.900
R-factor	0.217
Rwork	0.217
R-free	0.27100
Structure solution method	MAD
RMSD bond length	0.009
RMSD bond angle	25.300 *
Data reduction software	MOSFLM
Data scaling software	CCP4
Phasing software	X-PLOR (3.8)
Refinement software	X-PLOR (3.8)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	2.000
High resolution limit [Å]	1.900	1.900
Rmerge	0.054 *	0.226 *
Number of reflections	19245
<I/σ(I)>	8.1	2.9
Completeness [%]	96.9	98.2
Redundancy	4.7	4.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	6.5	4 *	PROTEIN WAS CRYSTALLIZED FROM 20% PEG 800, 200 MM AMMONIUM SA SULPHATE, 100 MM SODIUM CACODYLATE, PH 6.5

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	15-20 (mg/ml)
2	1	reservoir	PEG8000	20-24 (%)
3	1	reservoir	glycerol	15 (%)
4	1	reservoir	ammonium sulfate	0.2 (M)
5	1	reservoir	sodium cacodylate	0.1 (M)