1DZB

Crystal structure of phage library-derived single-chain Fv fragment 1F9 in complex with turkey egg-white lysozyme

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	EMBL/DESY, HAMBURG BEAMLINE X31
Synchrotron site	EMBL/DESY, HAMBURG
Beamline	X31
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-07-15
Detector	MARRESEARCH
Spacegroup name	P 1 21 1
Unit cell lengths	38.710, 112.440, 80.000
Unit cell angles	90.00, 97.67, 90.00

Refinement procedure

Resolution	20.000 - 2.000
R-factor	0.225 *
Rwork	0.225
R-free	0.30600
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1jhl
RMSD bond length	0.015
RMSD bond angle	0.045
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.070
High resolution limit [Å]	2.000	2.000
Rmerge	0.139	0.196
Total number of observations	297921 *
Number of reflections	40240	3449 *
<I/σ(I)>	12.1	3.77
Completeness [%]	87.9	75.5
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5		A SOLUTION OF SCFV FRAGMENT 1F9/TEL COMPLEX IN A 1:1 RATION IN 5 MM TRIS/HCL PH 7.5, 0.1 M NACL WAS MIXED WITH 10% PEG4000, 0.1 M AMMONIUMACETAT AND 0.05 M SODIUMACETATE BUFFER PH 5.0 AND EQUILIBRATED AGAINST 30% PEG4000

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	5 (mg/ml)
2	1	drop	Tris-HCl	5 (mM)	pH7.5
3	1	drop	sodium acetate	0.05 (M)	pH5.0
4	1	drop	PEG4000	10 (%(w/v))
5	1	drop		0.1 (M)
6	1	drop	ammonium acetate	0.1 (M)
7	1	reservoir	PEG4000	30 (%(w/v))