1DZ9
Putative oxo complex of P450cam from Pseudomonas putida
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSLS BEAMLINE X12C |
| Synchrotron site | NSLS |
| Beamline | X12C |
| Temperature [K] | 96 |
| Detector technology | CCD |
| Collection date | 1997-10-15 |
| Detector | BRANDEIS |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 66.900, 61.700, 94.600 |
| Unit cell angles | 90.00, 90.30, 90.00 |
Refinement procedure
| Resolution | 19.000 - 1.900 |
| Rwork | 0.177 |
| R-free | 0.24800 |
| Structure solution method | OTHER |
| RMSD bond length | 0.014 |
| RMSD bond angle | 0.033 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Refinement software | REFMAC |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.000 | 1.850 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.100 * | 0.333 * |
| Number of reflections | 66465 | |
| <I/σ(I)> | 8.4 | 1.7 |
| Completeness [%] | 92.9 | 59.2 |
| Redundancy | 2.9 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.4 | 275 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 30 (mg/ml) | |
| 2 | 1 | reservoir | Tris-HCl | 50 (mM) | pH7.4 |
| 3 | 1 | reservoir | 250 (mM) | ||
| 4 | 1 | reservoir | DTE | 100 (mM) | |
| 5 | 1 | reservoir | camphor | 1 (mM) | |
| 6 | 1 | reservoir | PEG4000 | 27-30 (%) |
