Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-6A |
| Synchrotron site | Photon Factory |
| Beamline | BL-6A |
| Temperature [K] | 286 |
| Detector technology | IMAGE PLATE |
| Collection date | 1995-03-15 |
| Detector | FUJI BAS-2000 IMAGE PLATE |
| Spacegroup name | P 41 2 2 |
| Unit cell lengths | 52.650, 52.650, 100.630 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.500 - 1.750 |
| R-factor | 0.189 |
| Rwork | 0.189 |
| R-free | 0.20500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1ARN |
| RMSD bond length | 0.008 |
| RMSD bond angle | 26.100 * |
| Data reduction software | WEIS |
| Data scaling software | CCP4 |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 37.200 | 1.800 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Rmerge | 0.062 | 0.214 |
| Total number of observations | 34438 * | |
| Number of reflections | 12084 | |
| <I/σ(I)> | 6.5 | 3.1 |
| Completeness [%] | 82.4 | 53.6 |
| Redundancy | 2.8 | 1.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6 | 277 * | 0.1M KH2 PO4 PH 6.0, 60% SATURATED AMMONIUM SULPHATE |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 10 (mg/ml) | |
| 2 | 1 | drop | 0.1 (M) | ||
| 3 | 1 | reservoir | ammonium sulfate | ||
| 4 | 1 | reservoir | 0.1 (M) |
