1DYW

Biochemical and structural characterization of a divergent loop cyclophilin from Caenorhabditis elegans

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	ENRAF-NONIUS FR571
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 41 21 2
Unit cell lengths	60.608, 60.608, 123.124
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	15.000 - 1.800
R-factor	0.2152
R-free	0.28610
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cwa
RMSD bond length	0.007
RMSD bond angle	0.024
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	SHELXL-97

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	15.000	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.083 *	0.278 *
Number of reflections	21337
<I/σ(I)>	11.75	2.587
Completeness [%]	97.0	96.2
Redundancy	6.59

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	5.6	18 *	MPEG5000, SODIUM CITRATE, PH 5.6

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	3.5 (mg/ml)
2	1	drop	sodium citrate	50 (mM)
3	1	drop	mPEG5000	15.5 (%(w/v))
4	1	reservoir	sodium citrate	100 (mM)
5	1	reservoir	mPEG5000	31 (%)