1DYP
1,3-ALPHA-1,4-BETA-D-GALACTOSE-4-SULFATE-3,6-ANHYDRO-D-GALACTOSE 4 GALACTOHYDROLASE
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | ESRF BEAMLINE BM14 |
| Synchrotron site | ESRF |
| Beamline | BM14 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-06-16 |
| Detector | MARRESEARCH |
| Wavelength(s) | 0.8860, 0.9755, 0.9787 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 56.408, 61.120, 75.523 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 24.250 - 1.540 |
| R-factor | 0.178 |
| Rwork | 0.178 |
| R-free | 0.19300 |
| Structure solution method | MAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 26.100 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.9) |
| Refinement software | CNS (0.9) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 24.250 | 1.600 |
| High resolution limit [Å] | 1.540 | 1.540 |
| Rmerge | 0.030 * | 0.116 * |
| Total number of observations | 120447 * | |
| Number of reflections | 38205 | |
| <I/σ(I)> | 16.1 | 6.2 |
| Completeness [%] | 97.7 | 81.3 |
| Redundancy | 3.15 * | 2.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 7.2 * | 285 * | Michel, G., (1999) Acta Crystallogr.,Sect.D, D55, 918. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 6.5 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 200 (mM) | ||
| 4 | 1 | reservoir | sodium acetate | 0.1 (M) | |
| 5 | 1 | reservoir | PEG4000 | 30 (%) | |
| 6 | 1 | reservoir | 50 (mM) |
