1DYN

CRYSTAL STRUCTURE AT 2.2 ANGSTROMS RESOLUTION OF THE PLECKSTRIN HOMOLOGY DOMAIN FROM HUMAN DYNAMIN

Experimental procedure

Spacegroup name	I 4 2 2
Unit cell lengths	85.200, 85.200, 132.500
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	8.000 - 2.200
R-factor	0.2
Rwork	0.200
R-free	0.32000
RMSD bond length	0.010
RMSD bond angle	1.645
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
Number of reflections	12140
Completeness [%]	97.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, sitting drop *	6.5 *	18 *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	9 (mg/ml)
2	1	drop	sodium potassium phosphate	0.45 (M)
3	1	reservoir	sodium potassium phosphate	1.8 (M)