1DYK
Laminin alpha 2 chain LG4-5 domain pair
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SRS BEAMLINE PX9.6 |
| Synchrotron site | SRS |
| Beamline | PX9.6 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 1999-10-15 |
| Detector | ADSC QUANTUM 4 CCD |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 70.600, 111.500, 124.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 20.000 - 2.000 |
| R-factor | 0.233 |
| Rwork | 0.233 |
| R-free | 0.26100 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1qu0 |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.500 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | X-PLOR |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.110 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.056 | 0.139 |
| Number of reflections | 31704 | |
| Completeness [%] | 94.8 | 93.6 |
| Redundancy | 3.4 | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 8 * | pH 7.50 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 10 (mM) | |
| 3 | 1 | drop | 100 (mM) | ||
| 4 | 1 | reservoir | sodium HEPES | 100 (mM) | |
| 5 | 1 | reservoir | 2-propanol | 2-8 (%(v/v)) | |
| 6 | 1 | reservoir | PEG4000 | 19-23 (%(w/v)) | |
| 7 | 1 | reservoir | 10 (mM) |
