1DXX

N-terminal Actin-binding Domain of Human Dystrophin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ELETTRA BEAMLINE 5.2R
Synchrotron site	ELETTRA
Beamline	5.2R
Temperature [K]	100
Detector technology	IMAGE PLATE
Detector	MARRESEARCH
Spacegroup name	P 1
Unit cell lengths	59.690, 79.330, 81.950
Unit cell angles	61.08, 78.22, 70.54

Refinement procedure

Resolution	40.000 * - 2.600
R-factor	0.232 *
Rwork	0.232
R-free	0.26800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1qag
RMSD bond length	0.013
RMSD bond angle	0.043
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	AMoRE
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.690
High resolution limit [Å]	2.600	2.600
Rmerge	0.051	0.250
Number of reflections	36284	3448 *
<I/σ(I)>	12.7	2.6
Completeness [%]	95.4	91.6
Redundancy	1.7	1.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	7.4	18 *	1.55M AMMONIUM FORMATE, 0.1M HEPES 7.4, pH 7.40

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop	ammonium formate	1.55 (M)
3	1	drop	HEPES	0.1 (M)
4	1	reservoir	ammonium formate	1.55 (M)
5	1	reservoir	HEPES	0.1 (M)