1DXM

Reduced form of the H protein from glycine decarboxylase complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	1998-12-15
Spacegroup name	P 31 2 1
Unit cell lengths	56.410, 56.410, 135.170
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	40.000 - 2.600
R-factor	0.207 *
Rwork	0.204
R-free	0.26100 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1hpc
RMSD bond length	0.007
RMSD bond angle	25.000 *
Data reduction software	XDS
Data scaling software	SCALA
Phasing software	CNS (0.5)
Refinement software	CNS (0.5)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	40.000	2.740
High resolution limit [Å]	2.600	2.600
Rmerge	0.110 *	0.240 *
Number of reflections	7808
<I/σ(I)>	4.7	3
Completeness [%]	97.0 *	98.3
Redundancy	3	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion *	5.2	8 *	50 % AMMONIUM SULFATE, 100 MM TRIS MALEATE PH 5.2, 2 MM TCEP (TRIS CARBOXYETHYL PHOSPHINE)

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	reservoir	ammonium sulfate	2 (M)
2	1	reservoir	TCEP	10 (mM)
3	1	reservoir	Tris maleate	100 (mM)