1DWW
MURINE INDUCIBLE NITRIC OXIDE SYNTHASE OXYGENASE DIMER N-hydroxyarginine and dihydrobiopterin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRL BEAMLINE BL9-1 |
| Synchrotron site | SSRL |
| Beamline | BL9-1 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-02-15 |
| Detector | MARRESEARCH |
| Spacegroup name | P 61 2 2 |
| Unit cell lengths | 212.980, 212.980, 114.200 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 30.000 - 2.350 |
| R-factor | 0.249 |
| Rwork | 0.249 |
| R-free | 0.28800 |
| Structure solution method | OTHER |
| RMSD bond length | 0.009 |
| RMSD bond angle | 23.400 * |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (0.4) |
| Refinement software | X-PLOR |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.430 |
| High resolution limit [Å] | 2.350 | 2.350 |
| Rmerge | 0.074 * | 0.289 * |
| Total number of observations | 303449 * | |
| Number of reflections | 62465 | |
| <I/σ(I)> | 28.9 | 7.4 |
| Completeness [%] | 98.7 | 96.7 * |
| Redundancy | 4.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion * | 7.6 * | 4 * | pH 6.00 |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 17 (mg/ml) | |
| 10 | 1 | reservoir | 18-21 (%) | or ammonium sulfate | |
| 2 | 1 | drop | HEPPS | 40 (mM) | |
| 3 | 1 | drop | glycerol | 10 (%) | |
| 4 | 1 | drop | dithiothreitol | 1 (mM) | |
| 5 | 1 | drop | NHA | 4 (mM) | |
| 6 | 1 | drop | 2 (mM) | ||
| 7 | 1 | drop | 4-amino-H4B | 2 (mM) | |
| 8 | 1 | reservoir | MES | 50 (mM) | |
| 9 | 1 | reservoir | beta-octylglucoside | 50 (mM) |
