1DWU

Ribosomal protein L1

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU RUH3R
Temperature [K]	293
Detector technology	IMAGE PLATE
Detector	R-AXIS IV
Spacegroup name	P 21 21 21
Unit cell lengths	67.360, 69.980, 106.170
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	30.000 * - 2.700 *
R-factor	0.207
Rwork	0.207
R-free	0.25400 *
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1cjs
RMSD bond length	0.014
RMSD bond angle	2.703
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	X-PLOR (3.851)
Refinement software	X-PLOR (3.851)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000 *	2.770
High resolution limit [Å]	2.700 *	2.680
Rmerge	0.070 *	0.275 *
Number of reflections	14280 *
Completeness [%]	94.6 *	81.7 *
Redundancy	4.31	3.8 *

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *	8.5 *	277 *	10% PEG 4K, 40MM MGCL2, 50MM NAAC PH 5.5, 50MM NACL, AGAINST: 30% PEG 4K, 100 MM NAAC

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	25-30 (mg/ml)
2	1	drop	PEG4000	30 (%)	precipitant
3	1	drop	Tris-HCl	0.1 (M)	precipitant
4	1	drop		0.2 (M)	precipitant
5	1	reservoir	PEG4000	30 (%)
6	1	reservoir		0.1 (M)