1DW9

Structure of cyanase reveals that a novel dimeric and decameric arrangement of subunits is required for formation of the enzyme active site

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	1998-07-15
Detector	ARGONNE APS-1
Wavelength(s)	0.9465, 0.9793, 0.9795, 1.033, 1.078, 1.00
Spacegroup name	P 1
Unit cell lengths	76.340, 81.030, 82.300
Unit cell angles	70.30, 72.20, 66.40

Refinement procedure

Resolution	20.000 - 1.650
R-factor	0.15 *
Rwork	0.150
R-free	0.18900
Structure solution method	MAD
RMSD bond length	0.018
RMSD bond angle	0.033
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	REFMAC

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	1.680
High resolution limit [Å]	1.650	1.650
Rmerge	0.039 *	0.198 *
Total number of observations	524489 *
Number of reflections	187107
<I/σ(I)>	26.4	3.9
Completeness [%]	94.2	80.6
Redundancy	2.8	1.7

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.3	18 *	used microseeding *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	ammonium sulfate	2.1 (M)
2	1	drop		50 (mM)
3	1	reservoir	Tris-HCl	50 (mM)