1DVX
CRYSTAL STRUCTURE OF HUMAN TRANSTHYRETIN IN COMPLEX WITH DICLOFENAC
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 293 |
| Detector technology | IMAGE PLATE |
| Collection date | 1998-10-10 |
| Detector | MACSCIENCE |
| Spacegroup name | P 21 21 2 |
| Unit cell lengths | 43.140, 85.740, 64.540 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 8.000 - 2.000 |
| R-factor | 0.192 * |
| Rwork | 0.192 |
| R-free | 0.21500 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.260 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.000 |
| High resolution limit [Å] | 2.000 | 1.900 |
| Rmerge | 0.086 | 0.217 |
| Total number of observations | 72331 * | |
| Number of reflections | 16154 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 97.1 | 90.5 |
| Redundancy | 4.48 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.4 | 293 | potassium chloride potassium phosphate ammonium sulfate, pH 7.4, VAPOR DIFFUSION, HANGING DROP, temperature 293K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 5 (mg/ml) | |
| 2 | 1 | drop | 100 (mM) | ||
| 3 | 1 | drop | phosphate | 100 (mM) | |
| 4 | 1 | drop | ammonium sulfate | 1 (M) | |
| 5 | 1 | reservoir | ammonium sulfate | 2 (M) |
