1DVR
STRUCTURE OF A MUTANT ADENYLATE KINASE LIGATED WITH AN ATP-ANALOGUE SHOWING DOMAIN CLOSURE OVER ATP
Experimental procedure
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Detector technology | AREA DETECTOR |
| Collection date | 1991 |
| Detector | SIEMENS |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.800, 73.400, 118.800 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 10.000 - 2.360 |
| R-factor | 0.191 |
| Rwork | 0.191 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.700 |
| Data reduction software | XDS |
| Phasing software | X-PLOR (3.1) |
| Refinement software | X-PLOR (3.1) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.000 |
| High resolution limit [Å] | 2.360 |
| Rmerge | 0.058 |
| Number of reflections | 15793 |
| Completeness [%] | 90.0 |
| Redundancy | 4.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Vapor diffusion, hanging drop * | 6.3 * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | enzyme | 0.4 (mM) | |
| 2 | 1 | drop | AMPPCF2P | 2.5 (mM) | |
| 3 | 1 | drop | imidazole | 50 (mM) | |
| 4 | 1 | drop | 75 (mM) | ||
| 5 | 1 | drop | octyl-beta-glucoside | 0.05 (%) | |
| 6 | 1 | drop | PEG3350 | 21 (%) | |
| 7 | 1 | reservoir | imidazole | 50 (mM) | |
| 8 | 1 | reservoir | PEG3350 | 28 (%) |
