1DVO

THE X-RAY CRYSTAL STRUCTURE OF FINO, A REPRESSOR OF BACTERIAL CONJUGATION

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X12C
Synchrotron site	NSLS
Beamline	X12C
Temperature [K]	100
Detector technology	CCD
Detector	BRANDEIS - B1
Spacegroup name	P 21 21 21
Unit cell lengths	37.570, 38.726, 145.420
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	20.000 - 2.000
Rwork	0.197
R-free	0.22400
RMSD bond length	0.009 *
RMSD bond angle	1.350
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	CNS (0.9)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	20.000	2.050
High resolution limit [Å]	2.000	2.000
Rmerge	0.047	0.109
Number of reflections	14726
Completeness [%]	97.3	87
Redundancy	7.4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	4 *	drop consists of equal volume of protein and reservoir solutions *
1	VAPOR DIFFUSION, HANGING DROP	6.5 *	4 *	drop consists of equal volume of protein and reservoir solutions *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	drop		150 (mM)
3	1	drop	MES	50 (mM)
4	1	drop	beta-mercaptoethanol	0.1 (%(v/v))
5	1	drop	EDTA	1 (mM)
6	1	reservoir	PEG4000	12 (%)
7	1	reservoir	Tris-HCl	50 (mM)