1DVG
CRYSTAL STRUCTURE OF RAT HEME OXYGENASE-1 IN COMPLEX WITH HEME; SELELENO-METHIONINE DERIVATIVE, MUTATED AT M51T,M93L,M155L,M191L.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1999-05-02 |
| Detector | RIGAKU RAXIS IV |
| Spacegroup name | P 43 |
| Unit cell lengths | 54.853, 54.853, 187.558 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.480 - 2.200 |
| R-factor | 0.212 * |
| Rwork | 0.212 |
| R-free | 0.25800 |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.134 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.480 | 2.280 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.087 | 0.227 |
| Total number of observations | 69625 * | |
| Number of reflections | 26622 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 95.1 | 89.8 |
| Redundancy | 2.62 | 2.28 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | Omata, Y., (1998) Acta Crystallogr., Sect.D, 54, 1017. * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 25 (mg/ml) | |
| 2 | 1 | drop | potassium phosphate | 50 (mM) | |
| 3 | 1 | reservoir | Tris | 0.1 (M) | |
| 4 | 1 | reservoir | PEG4000 | 30 (%) | |
| 5 | 1 | reservoir | sodium acetate | 0.2 (M) |
