1DVF

IDIOTOPIC ANTIBODY D1.3 FV FRAGMENT-ANTIIDIOTOPIC ANTIBODY E5.2 FV FRAGMENT COMPLEX

Experimental procedure

Detector technology	IMAGE PLATE
Detector	RIGAKU
Spacegroup name	C 1 2 1
Unit cell lengths	152.800, 79.400, 51.500
Unit cell angles	90.00, 100.20, 90.00

Refinement procedure

Resolution	7.000 - 1.900
R-factor	0.194
Rwork	0.194
R-free	0.23500 *
RMSD bond length	0.016
RMSD bond angle	2.000
Data reduction software	XENGEN
Phasing software	X-PLOR
Refinement software	X-PLOR

Data quality characteristics

	Overall
High resolution limit [Å]	1.900 *
Rmerge	0.085
Total number of observations	105969 *
Number of reflections	41404
Completeness [%]	82.5
Redundancy	2.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	Vapor diffusion, hanging drop *			Goldbaum, F.A., (1994) J.Mol.Biol., 241, 739. *

Crystallization Reagents in Literatures

ID	crystal ID	solution	reagent name	concentration (unit)	details
1	1	drop	protein	10 (mg/ml)
2	1	reservoir	PEG8000	15 (%(w/v))
3	1	reservoir	ammonium sulfate	0.2 (M)
4	1	reservoir	HEPES	0.1 (M)