1DVE
CRYSTAL STRUCTURE OF RAT HEME OXYGENASE-1 IN COMPLEX WITH HEME
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL41XU |
Synchrotron site | SPring-8 |
Beamline | BL41XU |
Temperature [K] | 100 |
Detector technology | IMAGE PLATE |
Collection date | 1998-10-07 |
Detector | RIGAKU RAXIS IV++ |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 56.139, 56.139, 183.984 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 27.750 - 2.400 |
R-factor | 0.214 * |
Rwork | 0.214 |
R-free | 0.26800 |
RMSD bond length | 0.006 |
RMSD bond angle | 1.086 |
Data reduction software | DENZO |
Data scaling software | SCALEPACK |
Phasing software | CNS |
Refinement software | CNS |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 27.750 | 2.440 |
High resolution limit [Å] | 2.400 | 2.400 |
Rmerge | 0.063 | 0.174 |
Total number of observations | 69033 * | |
Number of reflections | 12148 | |
<I/σ(I)> | 16.7 | |
Completeness [%] | 98.7 | 95.5 |
Redundancy | 5.68 | 4.05 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7 * | 293 | Omata, Y., (1998) Acta Crystallogr., Sect.D, 54, 1017. * |
Crystallization Reagents in Literatures
ID | crystal ID | solution | reagent name | concentration (unit) | details |
1 | 1 | drop | protein | 25 (mg/ml) | |
2 | 1 | drop | potassium phosphate | 50 (mM) | |
3 | 1 | reservoir | Tris | 0.1 (M) | |
4 | 1 | reservoir | PEG4000 | 30 (%) | |
5 | 1 | reservoir | sodium acetate | 0.2 (M) |