1DV8
CRYSTAL STRUCTURE OF THE CARBOHYDRATE RECOGNITION DOMAIN OF THE H1 SUBUNIT OF THE ASIALOGLYCOPROTEIN RECEPTOR
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | ELLIOTT GX-20 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 1997-05-25 |
| Detector | MARRESEARCH |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 113.720, 32.460, 40.820 |
| Unit cell angles | 90.00, 91.29, 90.00 |
Refinement procedure
| Resolution | 19.350 - 2.300 |
| Rwork | 0.195 |
| R-free | 0.24700 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 24.200 * |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | AMoRE |
| Refinement software | CNS (0.5) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 20.000 | 2.320 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Rmerge | 0.093 | 0.317 |
| Number of reflections | 7342 | |
| <I/σ(I)> | 7.2 | |
| Completeness [%] | 95.9 | 95.9 |
| Redundancy | 2 | 2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.8 * | 298 | drop consists of equal volume of protein and reservoir solutions * |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | drop | protein | 7.8 (mg/ml) | |
| 2 | 1 | drop | Tris-HCl | 20 (mM) | |
| 3 | 1 | drop | 120 (mM) | ||
| 4 | 1 | drop | 2 (mM) | ||
| 5 | 1 | drop | lactose | 20 (mM) | |
| 6 | 1 | reservoir | PEG8000 | 25 (%) | |
| 7 | 1 | reservoir | ammonium sulfate | 80 (mM) | |
| 8 | 1 | reservoir | sodium cacodylate | 100 (mM) |
