1DV2
The structure of biotin carboxylase, mutant E288K, complexed with ATP
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU RU200 |
| Temperature [K] | 273 |
| Detector technology | AREA DETECTOR |
| Collection date | 1999-10-07 |
| Detector | SIEMENS HI-STAR |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 81.300, 115.500, 122.400 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.500 |
| R-factor | 0.172 |
| Rwork | 0.171 |
| R-free | 0.20300 |
| RMSD bond length | 0.012 |
| RMSD bond angle | 2.350 |
| Data reduction software | SAINT |
| Data scaling software | SAINT |
| Phasing software | AMoRE |
| Refinement software | TNT (5E) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.610 |
| High resolution limit [Å] | 2.500 | 2.500 |
| Rmerge | 0.072 | 0.297 |
| Total number of observations | 82537 * | |
| Number of reflections | 37574 | 3543 * |
| <I/σ(I)> | 9.6 | |
| Completeness [%] | 92.6 | 73.1 |
| Redundancy | 2.2 | 1.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | Microdialysis * | 7 * | 4 * | PEG-8000 ATP magnesium chloride HEPPS potassium chloride, pH 7.5, VAPOR DIFFUSION, HANGING DROP, temperature 298K |
Crystallization Reagents in Literatures
| ID | crystal ID | solution | reagent name | concentration (unit) | details |
| 1 | 1 | 1 | protein | 10 (mg/ml) | |
| 2 | 1 | 2 | potassium phosphate | 10 (mM) | |
| 3 | 1 | 2 | EDTA | 1 (mM) | |
| 4 | 1 | 2 | dithiothreitol | 2 (mM) | |
| 5 | 1 | 2 | 1 (mM) |
